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in situ hybridization

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in situ hybridization [‚in ‚si·chü ‚hī·brəd·ə·zā·shən]
(cell and molecular biology)
A technique permitting identification of particular deoxyribonucleic acid or ribonucleic acid sequences while these sequences remain in their original location in the cell. A cell or tissue is treated with a fixative and then exposed to a labeled (by radioactivity or fluorescence) single-stranded nucleic acid probe that hybridizes with the targeted nucleic acid sequence, revealing its location on a chromosome band when the hybridized sequence is analyzed microscopically.


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With RISH, rapid in situ hybridization, a chromogenic ISH technology, the proprietary DNA probe technology ensures high specificity and accuracy for the mRNA target in a simple two-hour, five-step procedure.
Polymerase chain reaction and in situ hybridization for selected mRNAs followed in the early 1990s.
The remainder of the book contains review and research articles from scholars from Europe, Asia, and South America on topics such as mixtures of microorganisms in biocontrol, fluorescence in situ hybridization in aquatic bacteria, and improving fecal coliform removal and maturation ponds.
 
 
 
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