in situ hybridization


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Related to in situ hybridization: Fluorescent in situ hybridization

in situ hybridization

[‚in ‚si·chü ‚hī·brəd·ə·zā·shən]
(cell and molecular biology)
A technique permitting identification of particular deoxyribonucleic acid or ribonucleic acid sequences while these sequences remain in their original location in the cell. A cell or tissue is treated with a fixative and then exposed to a labeled (by radioactivity or fluorescence) single-stranded nucleic acid probe that hybridizes with the targeted nucleic acid sequence, revealing its location on a chromosome band when the hybridized sequence is analyzed microscopically.
References in periodicals archive ?
Cytogenetic and fluorescence in situ hybridization investigation of ring chromosomes characterizing a specific pathologic subgroup of adipose tissue tumors.
Determination of HER2/neu Status: A Pilot Study Comparing HER2/neu Dual In Situ Hybridization DNA Probe Cocktail Assay Performed on Cell Blocks to Immunohistochemisty and Fluorescence In Situ Hybridization Performed on Histologic Specimens.
2] Nonstandard abbreviations: HER2, human growth factor receptor 2; IHC, immunohistochemistry; UK NEQAS-ICC, United Kingdom National External Quality Assessment Scheme for Immunocytochemistry; FISH, fluorescence in situ hybridization.
Characterization of Aequipecten opercularis (Bivalvia: Pectinidae) chromosomes by different staining techniques and fluorescent in situ hybridization.
Rapid diagnostic of bacterial meningitis by real-time PCR and fluorescent in situ hybridization.
Discriminating the ancestral progenitors of hexaploid Festuca arundinacea using genomic in situ hybridization.
With in situ hybridization the proble is applied directly to a tissue section.
This agreement provides Affymetrix with an important opportunity to expand its QuantiGene ViewRNA Assay offering into the pathology and cancer research markets by providing new multiplex chromogenic in situ hybridization (CISH) and fluorescence in situ hybridization (FISH) RNA biomarker assays which are validated on clinical samples for research applications.
1) These guidelines included specific recommendations for HER2 immunohistochemistry (IHC) as well as in situ hybridization (ISH) in formalin-fixed, paraffin-embedded (FFPE) tissues.
The CISH method has shown a high concordance with fluorescent in situ hybridization (FISH)--based assays for the identification of HER2 gene amplification, now considered the standard for confirming eligibility to receive the drug," Dr.
Indirect detection methods discussed in the opening six contributions include nonradioactive northern and southern analyses from plant samples, direct in-gel hybridization of DNA with digoxigenin-labeled probes, and in situ hybridization of termite microbes.
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