In this study, we observed that culture supernatant of all SEPEC strains isolated from patients with sepsis (HC/UNICAMP, Campinas, SP, Brazil), (3) were cytotoxic to human umbilical vein endothelial cells (HUVEC), causing loss of intercellular junctions, cellular elongation and death.
The epithelial monolayer is sealed by an array of intra- and intercellular protein-protein interactions, which form intercellular junctions consisting of the apically localized tight junctions (TJs) along with the adjacent adherens junctions (AJ) and desmosomes .
Dr Lydia Tabernero explains the results, "Scientists had always thought the reason for these incredibly strong connections was because the molecules were very rigid and structured as they are in other, weaker intercellular junctions.
Tubes, layered sheets and patterned structures have been bioprinted, yielding 3-D tissues that are free of biomaterial scaffolding and characterized by tissue-like microarchitecture, including the development of intercellular junctions and endothelial networks.
All the intercellular junctions described are responsible for ensuring epithelial tissue stability, which means that for the EMT to initiate, the epithelia must lose the cell-cell and cell-basal lamina junction complexes (23) and, thus, their polar architecture.
Positivity was defined as distinct, condensed, continuous immunoreactivity localized to the intercellular junctions of keratinocytes, in essentially the same pattern as that seen in immunofluorescence studies.