ISTN3X; 96% identical), comprising one of Tn3-like inverted repeats
and putative coding regions for transposase, resolvase (also called repressor), and ampicillin resistance.
Different oligonucleotides with inverted repeats
were designed to form hairpin loop structures to test efficiency of the sodium bisulfite reaction method.
The amplification products are stem-loop DNA structures with several inverted repeats
of the target and cauliflower-like structures with multiple loops, yielding >500 [micro]g/mL.