ion-exchange chromatography


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ion-exchange chromatography

[′ī‚än iks‚chānj ‚krō·mə′täg·rə·fē]
(analytical chemistry)
A chromatographic procedure in which the stationary phase consists of ion-exchange resins which may be acidic or basic.
References in periodicals archive ?
Crude enzyme sample showed a number of smeared bands while three bands and one band was obtained after gel filtration and single band after ion-exchange chromatography, respectively (Figure-4).
Selection of a suitable mobile phase for the speciation of four arsenic compounds in drinking water samples using ion-exchange chromatography coupled to inductively-coupled plasma-mass spectrometry.
In contrast to ion-exchange chromatography and boronate-affinity methods, which can sometimes detect Hb variants, the "closed tube" principle of immunoturbidimetric assays does not allow the user to recognize spurious alterations in Hb elution profiles or patterns.
Several dedicated commercial amino acid analyzers have been in existence that use this original procedure in which amino acids are separated by ion-exchange chromatography followed by postcolumn derivatization with ninhydrin and detection by ultraviolet-visible spectrophotometry.
As with IEF, resolution of Trf isoforms by ion-exchange chromatography is based on charge and is not affected by neutral monosaccharide moieties.
TABLE 66 SALES OF REAGENTS IN ION-EXCHANGE CHROMATOGRAPHY, THROUGH 2017 ($
Physiological amino acids have been determined extensively in clinical laboratories by automated ion-exchange chromatography in combination with postcolumn ninhydrin detection (3, 4).
TABLE 92 SALES OF REAGENTS IN ION-EXCHANGE CHROMATOGRAPHY, THROUGH 2017 ($
A similarity between an ESIMS-based assay for [beta] chain glycation and ion-exchange chromatography for HbA1c values, in particular for concentrations between 6% and 10%, has also been observed (4).
All HPLC methods based on ion-exchange chromatography showed higher HbA1c percentages in the sample of the uremic patient.
1c] measurements by as much as 10-20% when ion-exchange chromatography is used, but has a negligible effect in boronate affinity chromatography [5].
Between the Bio-Rex 70 HPLC method used as a designated comparison method for the internal standardization of the DCCT study [2] and the Mono S ion-exchange chromatography method [20,21], differences as large as 20% were found at the reference range concentrations, despite both methods' claiming to measure Hb [A.