isoelectric focusing


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Related to isoelectric focusing: isoelectric point

isoelectric focusing

[¦ī·sō·i′lek·trik ′fō·kəs·iŋ]
(physical chemistry)
Protein separation technique in which a mixture of protein molecules is resolved into its components by subjecting the mixture to an electric field in a supporting gel having a previously established pH gradient. Also known as electrofocusing.
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Dunnett's test was used to determine if the number of eggs laid per female on each of the diet treatments supplemented with the isoelectric focusing fractions was significantly greater than the number of eggs laid per female on the Diet (control).
A small piece of muscle tissue was removed from each individual fillet, labelled, and stored at -70 [degrees] C prior to isoelectric focusing.
This report describes the use of isoelectric focusing (IEF) to document the natural occurrence of a M.
Determinations of A1AT phenotype have typically been performed via inspection of isoelectric focusing gels.
Isoelectric focusing (IEF) on agarose gels is an electrophoretic technique with excellent resolution and can be used to separate different Hb fractions and variants and globin chains.
The use of analytical isoelectric focusing for detection and identification of betalactamases.
1c] was done with a capillary isoelectric focusing system, previously described, (9) with a normal reference range of 5.
Scientists are applying techniques such as in silico computational analysis, isoelectric focusing, one and two-dimensional gel electrophoresis, high-performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR), and mass spectrometry (MS) to better understand the structure and functional interactions of cellular membrane proteins, leading to the design of more effective therapeutics for many human diseases.
ProteinSimple offers a family of instruments for automated Western blotting analysis as well as devices for isoelectric focusing of proteins and gel imaging.
Phenotyping uses isoelectric focusing (IEF) gel electrophoresis to separate the serum proteins based on charge.
Isoelectric focusing (IEF) showed that both isolates produced several [beta]-lactamases (Table), including a common enzyme with ESBL activity (isoelectric point [pI] 6.
Isoelectric focusing techniques allow reliable detection of exogenous EPO in urine.