isosbestic point

(redirected from isosbestic points)
Also found in: Medical.

isosbestic point

[‚ī·səs‚bes·tik ′point]
(physical chemistry)
During a chemical reaction, a point in the absorption spectrum (that is, a wavelength) where at least two chemical species (for example, reactant and product) have identical molar absorption coefficients, which remain constant as the reaction proceeds. A stable isosbestic point is evidence that a reaction is proceeding without forming an intermediate or multiple products.
References in periodicals archive ?
Furthermore, spectroscopic titration studies for the interaction of these food additives with DNA showed that these dyes bind to calf thymus DNA and distinct isosbestic points are observed clearly suggesting binding of the dyes to DNA.
The retina is illuminated through the pupil, preferably at isosbestic points for oxy- and deoxyhemoglobin, using several discrete visible/NIR light bands while the reflected light from vessels overlying the optic disk is analyzed.
One chooses a combination of absorption maxima and isosbestic points, with a total number of wavelengths equal to the number of components of interest.