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ligase |
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ligase [′lī‚gās] (biochemistry) An enzyme that catalyzes the union of two molecules, involving the participation of a nucleoside triphosphate which is converted to a nucleoside diphosphate or monophosphate. Also known as synthetase. How to thank TFD for its existence? Tell a friend about us, add a link to this page, add the site to iGoogle, or visit webmaster's page for free fun content. |
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| The presence of tuberculous bacilli was confirmed by ligase chain reaction (LCR) assay. superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, [gamma]-glutamate cysteine ligase, glutathione S-transferase, NADPH quinone oxidoreductase, heme oxygenase 1) via a genetic response pathway that acquires transcriptional activation of the antioxidant response element (ARE) by the transcription factor nuclear regulatory factor 2 (Nrf2) (Li et al. Five microliters of DNA digest was used in a ligation reaction containing 130 [micro]g/mL Bg/II adaptor-oligonucleotide and 13 [micro]g/mL Csp6I adaptor-oligonucleotide (Invitrogen) (14), 10x T4 DNA ligase buffer, T4 DNA ligase (1 U/[micro]L) (Amersham Pharmacia), and TAC-buffer in a final volume of 20 [micro]L. |
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