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light microscope |
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| The exudate may then be collected and prepared for examination in one of three ways: 1) Draw lesion aspirates into the barrel of the needle with a tuberculin syringe and cap the needle (4); 2) touch a light microscope slide to the vesicle fluid; or 3) touch a 400-mesh, plastic-coated specimen grid directly to the base of the lesion (5). While this traditional approach to specimen preparation procedure is satisfactory for producing acceptable matrix microstructures free of preparation artifacts, it is not good for edge preservation, as edges are often rounded and out of focus when viewed with the light microscope. Analysis of the light microscope pictures indicated that the degree of dispersion and the particle-size of the dispersed phase (i. |
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