luciferase


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Related to luciferase: luciferin

luciferase

[lü′sif·ə‚rās]
(biochemistry)
An enzyme that catalyzes the oxidation of luciferin.
References in periodicals archive ?
The readings of luminance intensity were equalized by the protein concentration of lysates, and the data indicated to the luciferase activities of the samples.
The luciferase assay substrate was purchased from Promega (Madison, WI, USA), and sodium chromate (N[a.
Luciferase is inactivated by the chemical reactions involved in direct labeling of antigens or antibodies (9).
Tavare of the University of Bristol in England and his colleagues reported in the August 1995 Current Biology that injected luciferase plasmids enabled them to monitor gene activity in single living cells taken from a hamster cell line.
2010) and second by evaluating these extracts for their anti-inflammatory and cytotoxic activities using a previously reported NF-KB luciferase and MIT assay (Johnson et at.
In addition, the expressed enzyme and the gene for the bioluminescent luciferase class of enzymes have considerable potential as labels for nucleic acid assays.
Through adept genetic manipulation, researchers have now taken the DNA that makes luciferase and transferred it into plant, insect, and other species that don't naturally light up.
a leading provider of products for studying the regulatory elements in the human genome, today announced the launch of the innovative LightSwitch Luciferase Assay System[TM], a comprehensive reporter assay solution for measuring transcriptional activation and translational efficiency in living cells.
Values for extract-treated cells were considered as hits if firefly luciferase activity was >125% and Renilla luciferase activity <125% of solvent-treated cells and if at least for four out of five experiments effect sizes were similar.
In contrast, atrazine failed to stimulate luciferase expression or to block the activation of ER[alpha] by E2 (Figure 1A-C).
The PCR fragments contain each of the alleles were cloned into the pGL-3 basic luciferase reporter vector (Promega, Madison, WI, USA).
By cloning the 3'UTR of TET2 containing these sites downstream of the firefly luciferase gene in the pMIR-REPORT reporter vector, the resultant plasmid was able to be co-transfected with a mimic of miR-29 or a scrambled miRNA control.