luciferin


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luciferin

[lü′sif·ə·rən]
(biochemistry)
A species-specific pigment in many luminous organisms that emits heatless light when combined with oxygen.
References in periodicals archive ?
But researchers have found that they can use luciferin and luciferase together to look for ATP as an indication of life.
Human Acronym Preparation Substrate End point Reference receptor used measured Aryl AhR Recombinant Luciferin Luminescence Han et al.
When the researchers sprayed luciferin onto their luciferase-producing weeds, the plants emitted a green glow in step with the circadian rhythm of the promoter: The light starts building in the predawn hours, peaks in the morning, and subsides as night approaches.
Human lung cancer cells engineered to generate light upon exposure to the chemical luciferin (A549-Luc cells) were injected into the tail vein of mice.
The plate was placed in a Lmax luminometer (Molecular Devices Corporation, Sunnyvale, CA, USA), and the following sequence of events was programmed for each well: a) injection of 100 [micro]L luciferin assay mix (Promega); b) 4-sec delay; c) light production measured over a 10-sec period.
For this model Daudi cells were transfected with luciferase which makes them bioluminescent after administration of luciferin, thus permitting detection using a sensitive camera.
Different enzymes, known collectively as luciferases, cut luciferin in different places, producing various colors of light.
Luciferin and isopropylthiogalactopyranoside were purchased from Promega (Charbionnieres, France).
When all three are present, luciferin reacts with ATP and emits light.
Injector M connected to lysis buffer and injector P connected to the luciferin substrate were set to inject 25 [micro]l of each solution into appropriate wells.
Animals were injected subcutaneously with luciferin (150 [micro]L, 30 mg/mL).
2+], catalyzes the oxidation of luciferin by molecular oxygen, yielding the excited state of oxyluciferin (2-4).