While the other studies used only the presence of the malaria parasite as the diagnostic criterion, this study used the malaria parasite and/or malaria pigment
, so that past or chronic malaria, which are more likely to be associated with structural and functional changes in placenta than acute malaria, could also be diagnosed.
For each histological section per animal not <ten microscopic fields were randomly selected and examined for any histopathological changes, such as malaria pigment
deposition and sequestered RBCs.
crystals as magnetic micro-rotors: key for high-sensitivity diagnosis.
2 mmol/L) Acidosis (plasma bicarbonate <15 mmol/L or serum lactate >5 mmol/L) Hepatic transaminases >3 times normal Renal impairment (serum creatinine >265 [micro]mol/L or rapidly rising creatinine or urine output <400 mL/day in an adult) Haemoglobin <5 g/L Parasitaemia [greater than or equal to] 5% [greater than or equal to] 5% neutrophils contain malaria pigment
Presence of schizonts of P.
Also phagocytosis of Malaria pigment
in acute Malaria by monocytes is seen.
Hemozoin or malaria pigment
is a crystalline form of free heme.
Association of intraleukocytic Plasmodium falciparum malaria pigment
with disease severity, clinical manifestations, and prognosis in severe malaria.
Presence of falciparum schizonts and/or malaria pigment
in 5% or more of neutrophils on a blood film are considered to be poor prognostic signs (12,77,78).
Sometimes parasites cannot be found in peripheral blood smear from patients with malaria, but malaria pigment
may be seen in circulating phagocytic leucocytes.
Two independent pathologists pooled and read histology slides (2 each) in a blinded fashion using light microscopy (at x40) and under polarized light to assess the deposition of malaria pigment
The paper entitled "Quinoline Binding Site on Malaria Pigment
Crystal: A Rational Pathway for Anti-Malaria Drug Design" is also available through http://pubs.
vivax has been inconsistently associated with the presence of malaria pigment
in the placenta, but not associated with placental pathologic changes (14,31).