melting profile

melting profile

[′melt·iŋ ‚prō‚fīl]
(biochemistry)
A plot of the degree of denaturation of the strands in a nucleic acid duplex in a specified time as a function of temperature.
References in periodicals archive ?
After COLD-PCR amplification, the melting profile shapes changed but remained clearly distinguishable from the wild-type control samples (Fig.
Melting curves and difference plots clearly showed a biphasic melting profile for samples from healthy individuals, representing the unmethylated and methylated SNRPN alleles.
Of the 18 samples with sequence variations in the amplicon, only 1 sample had an amplicon melting profile that was altered by >0.
The dynamic melting profile for the 96-well and 384-well heat blocks are shown in Fig.
To differentiate R67X and R67Q, all samples exhibiting a melting profile other than wild type were subjected to an RFLP analysis.
With the melting profile of Citrobacter freundii as a reference, we generated difference plots of these 25 bacterial species after fluorescence normalization and temperature overlay to superimpose the curves at 20%-60% fluorescence.
The least different but still distinctive melting profile was with the mutation K695R, which had a [T.
A tumor sample melting profile was considered aberrant if, when compared with the wild-type profile, the [T.
b) We estimated the melting profile by serial injections of PCR products at different column temperatures.
The Wavemaker software gives a computer-assisted determination of melting profile and analytical conditions for each DNA fragment.
The Plasma Thermogram[TM] (pT[TM]) technology is a robust diagnostic platform utilizing Differential Scanning Calorimetry (DSC) to generate a temperature-induced melting profile of the major blood proteins.