After COLD-PCR amplification, the melting profile
shapes changed but remained clearly distinguishable from the wild-type control samples (Fig.
Melting curves and difference plots clearly showed a biphasic melting profile
for samples from healthy individuals, representing the unmethylated and methylated SNRPN alleles.
Of the 18 samples with sequence variations in the amplicon, only 1 sample had an amplicon melting profile
that was altered by >0.
The dynamic melting profile
for the 96-well and 384-well heat blocks are shown in Fig.
To differentiate R67X and R67Q, all samples exhibiting a melting profile
other than wild type were subjected to an RFLP analysis.
With the melting profile
of Citrobacter freundii as a reference, we generated difference plots of these 25 bacterial species after fluorescence normalization and temperature overlay to superimpose the curves at 20%-60% fluorescence.
The least different but still distinctive melting profile
was with the mutation K695R, which had a [T.
A tumor sample melting profile
was considered aberrant if, when compared with the wild-type profile, the [T.
b) We estimated the melting profile
by serial injections of PCR products at different column temperatures.
The Wavemaker software gives a computer-assisted determination of melting profile
and analytical conditions for each DNA fragment.
The computations start (1) with melting profile
(AI) using Eqs.
The Plasma Thermogram[TM] (pT[TM]) technology is a robust diagnostic platform utilizing Differential Scanning Calorimetry (DSC) to generate a temperature-induced melting profile
of the major blood proteins.