Ana Colaco on the topic of Forecasting of Methanotrophs by methane monooxygenase
genes (sMMO) from the shallow water vent Espalamaca.
Polymerase chain reaction analysis revealed soluble methane monooxygenase genes in methanotrophic enrichments, and 16S rRNA analysis identified Methylocystis parvus with 98% similarity, further indicating the presence of a type II methanotroph.
A qualitative assay was performed using naphthalene and tetrazotized ortho-dianisidine to detect activity of soluble methane monooxygenase (sMMO) in the enriched methanotrophic-mixed cultures, as previously described (Bowman et al.
Optimization of trichloroethylene oxidation by methanotrophs and the use of a colorimetric assay to detect soluble methane monooxygenase activity.
Miguez adds, "the gene cluster cloning for an enzyme called soluble methane monooxygenase
(sMMO) is being cloned into Methylobacterium extorquens.
Extensive published research on enzyme active site structures such as those found in hemerythrin, ribonucleotide reductase, methane monooxygenase
and many other enzymes reveals the presence of binuclear metallic cores, many of which are carboxylate-bridged (refs.