methylate

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methylate

[′meth·ə‚lāt]
(organic chemistry)
References in periodicals archive ?
Low doses of a DNMT inhibitor have been found to alter tumorigenesis pathways and upregulate immunomodulatory pathways, and low-dose DNA methylation inhibitors have shown promise for treating leukemia.
For maintaining normal DNA methylation or for treatment, vitamin B12 and folate are known to facilitate DNA synthesis.
Skinner says that the articulation of epigenetics may take another three generations before studies such as those for DNA methylation become settled science.
3]), predominantly where cytosine bases occur consecutively, DNA methylation was first confirmed to occur in human cancer in 1983, and has since been observed in many other illnesses and health conditions.
In generics, imprinting describes the condition where one of the two alleles of a typical gene pair is silenced by an epigenetic process such as methylation or acetylation.
In studies published in the May--August 2004 issue of International Reviews of Immunology and the October 2003 issue of Clinical Immunology, he noted that pharmaceuticals such as the heart drug procainamide and the antihypertensive agent hydralazine cause lupus in some people, and demonstrated that lupus-like disease in mice exposed to these drugs is linked with DNA methylation alterations and interruption of signaling pathways similar to those in people.
In the present study, we designed a new primer set and assay conditions to determine the methylation status of PWS or AS patients in a rapid and closed-tube assay.
For MS-MA analysis, extracted genomic DNA was treated with sodium bisulfite and the EZ DNA Methylation Kit[TM] (Zymo Research) according to the manufacturer's protocol.
Genomic DNA was extracted from white blood cells according to standard phenol-chloroform procedures (13, 14) and then stored at 4[degrees]C until quantification of global DNA methylation.
The global DNA methylation status was assessed by use of the cytosine extension assay (16).
Preoperative serum AFP concentrations were measured by a two-site sandwich immunoassay (Automated Chemi-luminescence System 180; Bayer Diagnostics) and were correlated with the p16 methylation status in plasma or serum from the 45 HCC patients and the 35 non-HCC patients with chronic hepatitis/ cirrhosis.
We demonstrated the presence of methylated p16 sequences in plasma or serum from 80% (24 of 30) of HCC patients with tumoral p16 methylation (Table 1).