Spectrophotometry

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spectrophotometry

[¦spek·trō·fə′täm·ə·trē]
(analytical chemistry)
A method of chemical analysis based on the absorption or attenuation by matter of electromagnetic radiation of a specified wavelength or frequency. The radiation interacts with specific features of the molecular species being determined, such as the vibrational or rotational motions of the chemical bonds. The radiation can also interact with specific atoms or the whole molecule, for example, by causing the molecule to change its electronic energy state.

Spectrophotometry

 

a branch of measurement technology that draws on spectrometry, photometry, and metrology in developing methods and instruments for the quantitative measurement of spectral absorptance, reflectance, emissivity, and luminance as quantities characterizing media, coatings, surfaces, and radiators.

References in periodicals archive ?
Comparison and identification of automotive topcoats: Microchemical spot tests, microspectrophotometry, pyrolysis-gas chromatography, and diamond anvil cell FTIR, Canadian Society of Forensic Science Journal (1994) 27:209-223.
Microspectrophotometry and colorimetry are two analytical techniques for obtaining an objective color measurement.
Especially suited to the critical demands of microspectrophotometry, the new lenses transmit between 230 nm and 2,100 nm.
2013) identified a surprisingly large number of opsin transcripts in the eyes of stomatopods--up to 33 transcripts--that far outnumber physiologically distinct photoreceptor classes determined by microspectrophotometry or electrophysiology.
Once the samples are classified with reference to their probable source, a detailed study including microspectrophotometry and elemental analysis (Choudhry 1991) can be performed for a conclusive opinion.
UV absorption microspectrophotometry and digestibility of cell types of bermudagrass internodes at different stages of maturity.
These studies of the organization and dynamics of the root meristem are based on several lines of evidence, including observations on apical meristem cytohistological zonation, ultrastructure, cell cycle durations, mitotic indices, DNA microspectrophotometry, incorporation of radioactively labeled DNA precursors, and interpretations of cell lineages.
Cronin and Jinks (2001) reviewed structural and spectral aspects of crustacean visual ontogeny and predicted the presence of two receptor classes in some crustacean larvae despite the fact that microspectrophotometry has found only a single visual pigment.
Microspectrophotometry provides information about the ink's acting as a chemical mixture, but still does not provide the necessary detail that is important in component analysis for ink differentiation (Zeichner et al.
The low genome size variation observed in this study more closely agreed with the other flow cytometric studies than with the microspectrophotometry study.
Further study using intracellular recording or microspectrophotometry is needed to clarify all the spectral cell types.