Spectrophotometry

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spectrophotometry

[¦spek·trō·fə′täm·ə·trē]
(analytical chemistry)
A method of chemical analysis based on the absorption or attenuation by matter of electromagnetic radiation of a specified wavelength or frequency. The radiation interacts with specific features of the molecular species being determined, such as the vibrational or rotational motions of the chemical bonds. The radiation can also interact with specific atoms or the whole molecule, for example, by causing the molecule to change its electronic energy state.

Spectrophotometry

 

a branch of measurement technology that draws on spectrometry, photometry, and metrology in developing methods and instruments for the quantitative measurement of spectral absorptance, reflectance, emissivity, and luminance as quantities characterizing media, coatings, surfaces, and radiators.

References in periodicals archive ?
The test order is: microscopy, Fourier transform infrared spectroscopy (FTIR), microspectrophotometry or colorimetry, scanning electron microscopy/ energy dispersive spectroscopy (SEM-EDS), and, lastly, pyrolysis coupled with gas chromatography/mass spectrometry (GC/MS).
Infrared microscopy or, as it is more properly known, Fourier transform infrared microspectrophotometry (micro-FTIR) utilizing a Perkin-Elmer Auto Image System coupled to a Series 2000 FTIR.
Comparison of plant DNA contents determined by feulgen microspectrophotometry and laser flow cytometry.