The mobile phase
used for the elution was composed of 0.
Method development involves finding appropriate mobile phase
(organic and aqueous phase composition), stationary phase (specifications of the column), and flow characteristics such as sample injection volume and mobile phase
In such cases, the mobile phase
pH should be adjusted according to the pKa value of the compound, which can be challenging for mixtures of acidic and basic compounds and often necessitates screening buffered eluents of varying pH.
While for the mobile phase
was the type of solvent used, temperature, pH and flow rate in the system is handled .
In partition chromatography method, the stationary phase and the mobile phase
both are liquid.
Ions can also cause problems, by changing the ionic strength of the analyte in the mobile phase
and thereby resulting in adduct peaks.
TLC was done with 4 ^L of each solution and using a mobile phase
The method involves use of TLC plates coated with silica gel GF254 and a mixture of equal volumes of ethyl acetate and hexane as the mobile phase
Once the initial conditions of the mobile and stationary phases are fixed, further optimization should concentrate on less relevant parameters, such as the mobile phase
temperature and gradient profile, that could help improve the resolution .
The mobile phase
used to developed the separation of compounds was verified into seven combinations (hexane:ethyl acetate:methanol, chloroform:methanol:hexane, chloroform:methanol:water, ethyl acetate:methanol:hexane, diethyl ether:hexane, diethyl ether:hexane:methanol, acetone:methanol:water).
1% formic acid, was used in place of an isocratic mobile phase
of 5% acetonitrile-acetic acid at a ratio of 75:25.
The pH of the mobile phase
plays a decisive role in the efficiency of separations.