The mobile phase
temperature plays a key role for improving the peak shapes of proteins.
Table 1: Outline Control Strategy for Some of the Variables of an HPLC Separation Variable Control Noise Experimental (C) (N) (X) Column Packing type [check] Dimensions [check] Temperature [check] Mobile Phase
Organic % [check] Aqueous phase pH [check] Injection Volume [check] Vial temperature [check] Detector Wavelength [check]
In order to shorten column equilibration time and mitigate column degradation, each sample is run on the column of interest using the same mobile phase
before moving to a new mobile phase
Use guard columns to filter out potential interference contaminants from the SPE mobile phases
or separate the chromato grahic peak of the target analyte in the study samples from its interference peak in the HPLC mobile phases
Band 7 separated into seven secondary bands (5 visible and 2 UV absorbing) using petroleum ether: ethyl acetate (85: 15) as mobile phase
which were visualized under UV trans illuminator (Fig 3) .
As shown in Table 1, mobile phase
conditions and pH had a dramatic effect on the yield and purity of vancomycin.
The chromatographic tank was saturated with mobile phase
in the usual manner.
Solutions of venalafaxine HCl and its metabolite were prepared by dissolving weighed quantities in mobile phase
18] (5 [micro]m) column at 227 nm and using acetonitrile, methanol and water in the ratio of 60:5:35 (v/v) as a mobile phase
following single-step extraction from Phosphate buffer saline (PBS) pH 7.
A was 50% methanol, and mobile phase
B was methanol/isopropanol/water (75/20/5, vol/vol/vol), both containing 0.
The reverse-phase chromatographic assay uses an alkaline mobile phase
with UV detection.
Trapeze won the prestigious award for its introduction of Smart Mobile Phase
Two, which builds on and extends the company's original Smart Mobile solution that was launched last year.