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mutagenesis

   Also found in: Dictionary/thesaurus, Medical, Legal, Wikipedia, Hutchinson 0.09 sec.
mutagenesis
Genetics the generation, usually intentional, of mutations


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To optimize the assay, we performed PCR-based H274Y mutagenesis of the N1 fragment of the H5N1 virus (primers on request).
We used the QuickChange site-directed mutagenesis kit from Stratagene (La Jolla, CA, USA) and the following primers: 5'-GGTTCTGAATCAGTCTGAAATGCCTTCATTAAK4GC-3 and 5'-AATGAAGGCATTTCAGACTGATTCAGAACCAAACC-3'.
Nadeau is part of the International Mouse Mutagenesis Consortium, an effort to develop a mutant mouse strain for each of the 30,000 functional genes in the mouse genome.
 
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