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mutagen |
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mutagen: see mutation mutation, in biology, a sudden, random change in a gene , or unit of hereditary material, that can alter an inheritable characteristic. Most mutations are not beneficial, since any change in the delicate balance of an organism having a high level of adaptation to its ..... Click the link for more information. . mutagenAny agent capable of altering a cell's genetic makeup by changing the structure of the hereditary material, DNA. Many forms of electromagnetic radiation (e.g., cosmic rays, X rays, ultraviolet light) are mutagenic, as are various chemical compounds. The effects of some mutagens are increased or suppressed in some organisms by the presence of certain other, nonmutagenic substances; oxygen, for example, makes cells more sensitive to the mutagenic effects of X rays. |
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To optimize the assay, we performed PCR-based H274Y mutagenesis of the N1 fragment of the H5N1 virus (primers on request). We used the QuickChange site-directed mutagenesis kit from Stratagene (La Jolla, CA, USA) and the following primers: 5'-GGTTCTGAATCAGTCTGAAATGCCTTCATTAAK4GC-3 and 5'-AATGAAGGCATTTCAGACTGATTCAGAACCAAACC-3'. Nadeau is part of the International Mouse Mutagenesis Consortium, an effort to develop a mutant mouse strain for each of the 30,000 functional genes in the mouse genome. |
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