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mutagenesis |
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mutagenesis Genetics the generation, usually intentional, of mutations How to thank TFD for its existence? Tell a friend about us, add a link to this page, add the site to iGoogle, or visit webmaster's page for free fun content. |
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| To optimize the assay, we performed PCR-based H274Y mutagenesis of the N1 fragment of the H5N1 virus (primers on request). We used the QuickChange site-directed mutagenesis kit from Stratagene (La Jolla, CA, USA) and the following primers: 5'-GGTTCTGAATCAGTCTGAAATGCCTTCATTAAK4GC-3 and 5'-AATGAAGGCATTTCAGACTGATTCAGAACCAAACC-3'. Nadeau is part of the International Mouse Mutagenesis Consortium, an effort to develop a mutant mouse strain for each of the 30,000 functional genes in the mouse genome. |
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