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Cells were grown in phenol red-free EMEM containing 2 mM glutamine, 1% nonessential amino acids, 100 U/mL antibiotics (mix of penicillin, streptomycin, and fungizone), 1 mM sodium pyruvate, and 10% fetal calf serum (Biowhittaker, Gagny, France).
Casein is considered a complete, high quality protein that contains a full complement of the essential and nonessential amino acids, although it has been overshadowed by whey in the past despite its impressive profile.
3 Nonessential amino acids in serum Glutamine, [micro]mol/L 480.
In Buchnera, the amazing thing is that they keep all the pathways for the essential amino acids and lose all the nonessential amino acids that there is no shortage of [in the host]," says Moran.