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A deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) sequence composed of two or more covalently linked nucleotides. Oligonucleotides are classified as deoxyribooligonucleotides or ribooligonucleotides. Fragments containing up to 50 nucleotides are generally termed oligonucleotides, and longer fragments are called polynucleotides. See Deoxyribonucleic acid (DNA), Ribonucleic acid (RNA)

A deoxyribooligonucleotide consists of a 5-carbon sugar called deoxyribose joined covalently to phosphate at the 5 and 3 carbons of this sugar to form an alternating, unbranched polymer. A ribooligonucleotide consists of a similar repeating structure where the 5-carbon sugar is ribose. Chemically synthesized oligonucleotides of predetermined sequence have proven to be very useful for studying a large number of biochemical processes. In the 1960s, these compounds were used to decipher the genetic code. Later, chemically prepared deoxyoligonucleotides were joined to form genes for transfer RNAs. Gene synthesis from synthetic deoxyoligonucleotides is now routinely used to prepare genes and modified genes for proteins having potential clinical applications. Oligonucleotides have also been used to diagnose genetic disorders and bacterial or viral infections. See Gene, Genetic code, Genetic engineering, Nucleic acid


A polynucleotide of low molecular weight, consisting of less than 20 nucleotide polymers.
References in periodicals archive ?
Single-well genotyping of diallelic sequence variations by a two-color ELISA-based oligonucleotide ligation assay.
High-density multiplex detection of nucleic acid sequences: oligonucleotide ligation assay and sequence-coded separation.
More recently, detection techniques have utilized allele-specific oligonucleotide (A50) hybridization (3, 4), the single-stranded conformational polymorphism method (5), allele-specific priming of PCR (6, 7), primer-guided nucleotide incorporation assays (8), and oligonucleotide ligation assays (9).
Fin] mutation: a PCR-restriction fragment length polymorphism (PCR-RFLP) analysis and an oligonucleotide ligation assay (OLA).
These tests included restriction enzyme digestion, the introduction of new cutting sites, allele-specific PCR, oligonucleotide ligation assay, and single-strand conformation polymorphism (SSCP) analysis (1-5).
1] applied a DNA-based screening method, the oligonucleotide ligation assay (OLA), to determine the frequency of the A985G mutant allele in a Finnish population.
We have now combined PCR with a colorimetric oligonucleotide ligation assay (OLA) to detect the [A.
Many methods have been used to detect C282Y, including oligonucleotide ligation assays (1), fragment length polymorphism analyses of PCR products after restriction enzyme digestion (5), allele-specific oligonucleotide hybridization assays (2), mutagenically separated PCR assays (10), primer extension assays (11, 12), and allele refractory mutation systems (13, 14).
The strategies developed to screen HH chromosomes for the C282Y (845A) mutation include allele-specific oligonucleotide hybridization (4), restriction enzyme analysis (5), and oligonucleotide ligation assays (3).
Currently, several different Apo E genotyping techniques have been described; these include minisequencing (8), single-strand conformation polymorphism (9), allele-specific oligonucleotide probes (10), oligonucleotide ligation assays (11), restriction isotyping with HhaI or AflIII/HaeII (12,13), and the amplification refractory mutation system (ARMS) (14).