The peripheral pattern showed fluorescent signals distributed in the periphery of the oocyte; the semiperipheral pattern showed fluorescent signals covering most of the cytoplasmic volume without its central part; the diffuse pattern showed mitochondria distributed homogeneously throughout the ooplasm
The follicle was regarded as degenerated if it contained a cytoplasmic microvacuolation, pyknotic nucleus, shrunken ooplasm
, and/or disrupted granulosa cells (Figure 2C).
5% eosin staining revealed that the ooplasm
was homogeneous and held strong basophilic characteristics.
CoCs with two or more layer of granulosa cells and homogeneous granular ooplasm
were selected to IVM procedure .
Oocytes were graded by the morphological appearance of the cumulus cells and ooplasm
under zoom stereomicroscope (Chauhan et al.
The zygote centrosome has duplicated centrioles and maternal gamma tubulin is further added to the PCM in the ooplasm
to make it a functional centrosome, when the centrosome forms the sperm monoaster.
An example of such a technology that is already in use is ooplasm
transfer, a technique employed to circumvent problems in some women's eggs that involves replacing the nucleus of a donor's egg with the nucleus of another woman's egg (usually the intended mother's).
Developmental stage Characteristics EP The ooplasm
is strongly stained by haematoxylin.
REPRODUCTIVE SPECIALISTS: "Although human ooplasm
donation has been
Atretic oocytes are easily recognized in stained tissues by the separation of the oocyte membrane from the ooplasm
, or alternatively, separation of the ooplasm
from the nucleus.
By contrast the ooplasm
in the larger, terminal vitellogenic follicle is opaque due to the uptake of the dye.
After obtaining the histological preparations, ovarian stages and oocyte substages were determined through microscopic observations based on morphological characteristics of the ooplasm
, oolemma, and follicular epithelium surrounding the oocyte.