Besides deviations from the regular number of PNi and variations in their genetic constitution, there is a rare morphologic abnormality at the pronuclear stage; an early division of the ooplasm
that resembles embryonic cleavage.
Following the large number of small, clear vacuoles called yolk vesicles, appeared in the periphery of the ooplasm
It shows intense basophilic ooplasm
and the nucleus or germinal vesicle with several perinuclear nucleoli (Figure 2C).
Numerous degenerated follicles with cytoplasmic vacuolization, mitotic catastrophe in mitochondria , chromatin condensation, mitochondrial vesiculation and dispersion at ooplasm
2B and Table 2), with the ooplasm
filled with acidophilus spheric yolk globules (Figs.
The COCs were classified as grade A, B, C and D, on the basis of their cumulus investment and ooplasm
homogeneity (Sabasthin et al.
1) Sheldon Krimsky, Is Ooplasm
Transfer Safe for the Offspring, 27 GeneWatch 22 (2014).
As shown in Figure 2, pmTOR signals are observed as puncta in the ooplasm
(Sancak et al.
Barnabas Hospital in New Jersey transferred ooplasm
containing mitochondria from healthy donor eggs to the eggs of women experiencing infertility.
Also, a small round or oval darkly stained yolk nucleus was appeared in the ooplasm
near the nucleus.
Furthermore, only the phosphorylated, active form of MAPK was detected in GVs, although both active and inactive ERK1/2 were present in the ooplasm
after culture for 25 h (that is, just before GVBD).
sup],,, With respect to this problem, it was presumably related to the reduced cortical granules at the cortex of cryopreserved GV oocytes, as well as the appearance of vacuoles and small mitochondria-smooth endoplasmic reticulum complexes in the ooplasm