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open reading frame

   Also found in: Acronyms, Wikipedia 0.01 sec.
open reading frame [¦ō·pən ¦rēd·iŋ ¦frām]
(cell and molecular biology)
A stretch of triplets contained between an initator codon and a terminator codon. Abbreviated ORF.


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In addition, we investigated the possibility of amplifying the entire VP1 open reading frame by encoding the major capsid protein of MCPyV, using VP1F/VP1R primer sets (5'-CCTGAATTACAAGTAATTGAAGATGGCACC-3' and 5'-CTGAATAGGAATGCATGAAATAATTCTCAT-3', respectively).
Sequence analysis indicated that the tentatively named raven circovirus (RaCV) was 1898 nucleotides in size with 2 major open reading frames synonymous with other avian circoviruses, ORF C1 and ORF V1, likely to encode a putative capsid protein (Cap) and replicase-associated protein (Rep), respectively.
The programs available on this page include PCR Oligo Calculator, which lets researchers design batch polymerase chain reaction primers for amplifying the open reading frames for a given set of genes, and Batch Gene Retriever, which allows downloads of full-length sequence information from National Center for Biotechnology Information databases in batch mode.
 
 
 
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