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origin of replication |
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origin of replication [¦är·ə·jən əv ‚rep·lə′kā·shən] (cell and molecular biology) The nucleotide sequence from which deoxyribonucleic acid replication begins. How to thank TFD for its existence? Tell a friend about us, add a link to this page, add the site to iGoogle, or visit webmaster's page for free fun content. |
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| Since the origin of replication is a constant and conserved part of a plasmid, replicon typing focused on this portion of the plasmid is a more sensitive and specific method for identifying phylogenetically related plasmids than restriction-based analysis of the entire plasmid. The scientists used this technique to label an area near the origin of replication, a well-kn own site on bacterial chromosomes. Strains that define the W family have several distinguishing genotypes in common: 1) they belong to principal genetic group 1 (17,21); 2) they have similar spoligotype patterns, characterized by a deletion of spacers 1-34 and the corresponding repeat in the direct repeats (DR) region (19, 22-24), an alteration that defines spoligopattern S00034 and closely related spoligotypes; 3) they contain a unique insertion in the origin of replication (19,20) and in the NTF locus (20,25). |
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