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paper chromatography

   Also found in: Dictionary/thesaurus, Medical, Financial, Wikipedia, Hutchinson 0.03 sec.

paper chromatography (PC)

Type of chromatography using filter paper or other special paper as the stationary phase. Spots of sample and reference materials are applied, usually as liquids, near one edge (or corner, for two-dimensional PC) of the paper. The edge of the paper is dipped in a solvent, which travels along it by capillarity, moving the components of the sample at rates depending on their relative solubilities in the solvent. In two-dimensional PC, the paper is then turned 90° and the new edge dipped in a different solvent. The components of the sample mixture, visible as separated spots, are identified by comparing the distances they have traveled with those of the known reference materials. PC is especially useful for complex mixtures of amino acids, peptides, carbohydrates, steroids, and many other organic compounds and inorganic ions.

paper chromatography [′pā·pər ‚krō·mə′täg·rə·fē]
(analytical chemistry)
Procedure for analysis of complex chemical mixtures by the progressive absorption of the components of the unknown sample (in a solvent) on a special grade of paper.
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Flavonoids (I-III) were isolated from an ethanolic extract of aerial parts of Dryopteris villarii by preparative paper chromatography in BAW (n-butanolacetic acid-water, 4:1:5, upper phase), 15% AcOH (acetic acid) and BEW (n-butanol-ethanol-water, 4:1:2.
A New Flavone Glucoside, Apigenin 7-O-glucoside 4'-acetate and a New ... by Imperato, Filippo / American Fern Journal
The teacher assigns a paper chromatography experiment and has students memorize a definition for photosynthesis.
Less is more: trimming the overstuffed curriculum: through a science ... by Fratt, Lisa / District Administration
After the success of paper chromatography many researchers had tried to separate substances by electrophoresis in filter paper hanging in a chamber saturated with water vapor in a manner similar to that used with solvents in paper chromatography.
The Sephadex story by Flodin, Per / Polymer Engineering and Science
 
 
 
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