phosphorylation

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phosphorylation,

chemical process in which a phosphate group is added to an organic molecule. In living cells phosphorylation is associated with respirationrespiration,
process by which an organism exchanges gases with its environment. The term now refers to the overall process by which oxygen is abstracted from air and is transported to the cells for the oxidation of organic molecules while carbon dioxide (CO2
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, which takes place in the cell's mitochondria, and photosynthesis, which takes place in the chloroplasts. The energy released during metabolic or photosynthetic processes is captured in the energy-rich phosphate bonds of certain molecules, most commonly in the high-energy bonds of adenosine triphosphateadenosine triphosphate
(ATP) , organic compound composed of adenine, the sugar ribose, and three phosphate groups. ATP serves as the major energy source within the cell to drive a number of biological processes such as photosynthesis, muscle contraction, and the synthesis of
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 (ATP). In the process of oxidative phosphorylation ATP formation is associated with respiratory uptake of oxygen. In this process a cell substance known as NADH (one of a variety of coenzymescoenzyme
, any one of a group of relatively small organic molecules required for the catalytic function of certain enzymes. A coenzyme may either be attached by covalent bonds to a particular enzyme or exist freely in solution, but in either case it participates intimately in
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) donates hydride ions (one proton and two electrons) to the first of a series of enzymes in the so-called electron transport chain. As the hydride ion is passed from one enzyme to another in the chain, energy is made available to power the formation of ATP from adenosine diphosphate (ADP) and inorganic phosphate. At the end, or lowest energy level, of the electron transport chain, the hydride ion combines with oxygen and a proton (hydrogen ion) to form a water molecule. The phosphorylation process is linked to cell metabolism in that metabolic degradation of food, e.g., glucose, allows formation of the coenzyme NADH. The electron transport enzymes are complex aggregates of cytochromes, i.e., proteins with iron-containing heme groups, and various coenzymes. The precise mechanisms by which chemical energy is coupled to ATP synthesis are not yet understood. In photosynthetic phosphorylation, or photophosphorylation, substances such as the reduced coenzyme NADPH also donate hydride ions to an electron transport system so that ATP is synthesized from ADP and inorganic phosphate; in photophosphorylation, however, the coenzyme is produced from chemical reactions initiated by illuminated photosynthetic pigments instead of from metabolism of food molecules. The net result in phosphorylation of ADP is the formation of the high-energy molecule ATP, which the cell can use as a kind of universal energy currency to power many important cell processes, such as protein synthesis. Other phosphorylation reactions occur in cells, some without mediation by the electron transport chain, e.g., ATP is formed from ADP and inorganic phosphate in a reaction coupled to the oxidation of glyceraldehyde phosphate to phosphoglyceric acid.

Phosphorylation

 

the replacement of a hydrogen atom in a molecule of a chemical compound by an acid residue of phosphorus, usually a residue of phosphoric acid. Primary and secondary amines, alcohols, mercaptans, and other nucleophilic compounds are the most readily phosphorylated. Hydrocarbons (radical mechanism) and alkyl halides (ion mechanism) can also be subjected to phosphorylation. The phosphorylating agents used are acids of phosphorus and the derivatives of these acids, usually acid halides and anhydrides and, less often, esters and amides. For example:

ROH + CIP(O)(OR’)2

→ ROP(O)(OR’)2 + HCI

3R2NH + PCI3 → P(NR2)3 + 3HCI

During phosphorylation, the acids of phosphorus usually act together with condensing agents, such as carbodiimides and sulfochlorides. The capacity for phosphorylation depends on the valence of phosphorus in the phosphorylating agent, with derivatives of phosphorus (III) having the highest reactivity. Phosphorylation is widely used in the synthesis of noncombustible materials and of plasticizers, extractants, pesticides, and pharmaceuticals.

Phosphorylation has an important metabolic role in animals, plants, and microorganisms. It is catalyzed by enzymes and occurs as a result of either phosphorolysis or phosphokinase-catalyzed reactions:

A—B + C—H2PO3→A—H2PO3 + B—C

where A—B is the molecule that accepts the phosphoryl group (acceptor), and C—H2PO3 is the molecule that gives up the phosphoryl group (donor). Donors of the phosphoryl group include molecules of adenosine triphosphate and other nucleoside triphosphates. During metabolism, various low-molecular-weight compounds, as well as proteins, are subjected to phosphorylation. The phosphorylation of adenosine diphosphate by inorganic phosphoric acid serves as a basic mechanism for the formation of adenosine triphosphate and the accumulation of energy necessary for biosynthesis, as well as for the mechanical, electrical, and osmotic activity of the cell; it is carried out by polyenzymatic systems as a result of oxidation reactions of low-molecular-weight organic compounds either in the absence of oxygen (glycolytic phosphorylation) or through the effect of oxygen (oxidative phosphorylation). The phosphorylation of adenosine diphosphate into adenosine triphosphate during photosynthesis is called photophosphorylation.

E. E. NIFANTEV and A. D. VINOGRADOV

phosphorylation

[‚fäs‚fȯr·ə′lā·shən]
(organic chemistry)
The esterification of compounds with phosphoric acid.
References in periodicals archive ?
DAP phosphorylates via the same phosphorus-nitrogen bond breakage and under the same conditions as protein kinases, which are ubiquitous in present-day life forms," he said.
BTK, in concert with the protein tyrosine kinase Syk and the adaptor protein BLNK, has recently been demonstrated to phosphorylate and activate phospholipase C-r2 (PLC-r2).
As polymeric NFs are slowly transported within the axon, proline-directed kinases sequentially phosphorylate tail domain KSP repeat sites; this promotes further extension of sidearms and crossbridge formation between NFs and microtubules, which assemble into a stable cytoskeletal core.
2004) ATM and DNA-PK function redundantly to phosphorylate H2AX after exposure to ionizing radiation.
In contrast, he says, kinase molecules localized in dendritic spines could easily phosphorylate other kinase molecules within the same spine "and thereby form local bistable switches that are independent of the switches in other spines.
Consistent with these results, CGA was found to phosphorylate AMPK and ACC, consistent with the result of increased AMPK activities.
Each kinase domain can phosphorylate a substrate, and thus encode one bit of synaptic information.
In this study, we showed that a variety of protein kinases including PKC[alpha], PKA, CaMKII, and ERK1/2 phosphorylate the recombinantly expressed N-terminal tail of DAT (N-DAT) in vitro.
Phosphoaminoacid analysis of NDAT phosphorylated by PKC, PKA or CaMKII revealed that these kinases phosphorylate NDAT exclusively on serines.
ERKs and p38 kinase phosphorylate p53 protein at serine 15 in response to UV radiation.
The targets included focal adhesion kinase (FAK), Pyk2 (a soluble form of FAK), c-Src which can phosphorylate FAK, and the MAP kinases ERK1/2, p38 and JNK.
OSI) Prosidion is developing additional product candidates, including a Glucokinase Activator (in Phase I clinical trials) and a Glycogen Phosphorylate Inhibitor, PSN357 (in Phase IIa clinical trials).

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