After 24 h the supernatant of infected cells was recovered for evaluating viral titer by plaque assay
1A and B the proliferation of influenza virus was inhibited in the multiple cycles of influenza growth using plaque assay
and RT-PCR techniques.
RT-PCR and plaque assay
results were similar; detection rates did not differ among specific tissues or between field-collected vs.
In addition to RT-PCR technique we used TCID 50% and plaque assay
to assess the effect of PPE on influenza proliferation.
After centrifugation, the supernatant was 10-fold sequential diluted with MEM to determine virus titers by the plaque assay
with Hela cells (Bishop and Koch, 1969).
These 3 mixed infections were further plaque-purified as above and yielded single clonal populations of D or E, which validated our plaque assay
for isolating clonal populations.
Infectivity titers were determined by a standard plaque assay
on confluent RC-37 cells.
Specimens were tested for WNV-neutralizing antibodies by plaque-reduction neutralization test (PRNT) and for virus isolation by Vero cell plaque assay
(9) or WNV antigen by VecTest WNV Antigen Detection Assay (Medical Analysis Systems, Ventura, CA, USA).
Titration of the supernatants by plaque assay
gave titers of [10.
After 1, 10, 20, 30, 60 min and 2, 3, and 4 h, an aliquot was removed and assayed for remaining infectivity on confluent monolayers of RC-37 cells in 6 well plates by plaque assay
All CPE-positive samples were titrated by plaque assay
on Vero cells.
prowazekii inoculum was quantified by using either the plaque assay
method (17) or comparatively by 10-fold serial dilutions of a known plasmid standard of R.