Phospholipids in ram semen: metabolism of plasmalogen
and fatty acids.
Biochemical investigations like RBC plasmalogen levels and phytanic acid levels could not be done because of financial constraints.
2] Rhizomelic chondrodysplasia punctata is a disorder caused by abnormal peroxisomal function which can be mediated both through disorders of biosynthesis, for example, peroxisomal assembly (RDCP1) and by single enzyme defects affecting plasmalogen synthesis (RCDP2, RCDP3).
Peroxisomes are single-membrane-bounded ubiquitous organelles containing a hundred different enzymes that catalyze various metabolic pathways, including O-oxidation of very long-chain fatty acids, the synthesis of ether lipids such as plasmalogens, and bile-acid metabolism (1) (Table 1).
Two methods were developed for the isolation of mammalian somatic cell mutants defective in peroxisome biogenesis: (i) colony autoradiographic screening with a phenotypic marker, dihydroxyacetonephosphate acyltransferase (DHAP-ATase) deficiency; (28),(29) and (ii) the photo-sensitized selection method using 9(1,-pyrene)nonanol (P9OH) and an exposure to long wave-length ultraviolet (UV) light which kills wildtype cells incorporating P9OH as a fatty alcohol into plasmalogens and survive cell mutants deficient in such activity.
The abundance of plasmalogens in molluscs has been previously reported (Joseph 1982, Chapelle 1987, Kraffe et al.
The biological significance of plasmalogens in defense against oxidative damage.
, fatty acids and alkyl glyceryl ethers of marine and freshwater clams and mussels.
Total fatty acids, plasmalogens
, and fatty acid composition of ethanolamine and choline phosphoglycerides.
It mostly exists in the forms of phospholipids phosphatidylcholine (lecithin), lysophosphatidylcholine, choline plasmalogens
and sphingomyelin, and is widely distributed in all plant and animal cells (Zeisel, 1990).
In different aquatic ectothermal animals, it has also been reported that plasmalogens
increase in response to an elevation of temperature (Yeo et al.
Dimetylacetals (DMA) from PE plasmalogens
in the GC analyses were not identified.