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Related to Plasmid: Plasmid DNA


A circular extrachromosomal genetic element that is ubiquitous in prokaryotes and has also been identified in a number of eukaryotes. In general, bacterial plasmids can be classified into two groups on the basis of the number of genes and functions they carry. The larger plasmids are deoxyribonucleic acid (DNA) molecules of around 100 kilobase (kb) pairs, which is sufficient to code for approximately 100 genes. There is usually a small number of copies of these plasmids per host chromosome, so that their replication must be precisely coordinated with the cell division cycle. The plasmids in the second group are smaller in size, about 6–10 kb. These plasmids may harbor 6–10 genes and are usually present in multiple copies (10–20 per chromosome). See Gene

Plasmids have been identified in a large number of bacterial genera. Some bacterial species harbor plasmids with no known functions (cryptic plasmids) which have been identified as small circular molecules present in the bacterial DNA. The host range of a particular plasmid is usually limited to closely related genera. Some plasmids, however, are much more promiscuous and have a much broader host range.

The functions specified by different bacterial plasmids are usually quite specialized in nature. Moreover, they are not essential for cell growth since the host bacteria are viable without a plasmid when the cells are cultured under conditions that do not select for plasmid-specified gene products. Plasmids thus introduce specialized functions to host cells which provide versatility and adaptability for growth and survival. Plasmids which confer antibiotic resistance (R plasmids) have been extensively characterized because of their medical importance. Plasmids have played a seminal role in the spectacular advances in the area of genetic engineering. Individual genes can be inserted into specific sites on plasmids in cell cultures and the recombinant plasmid thus formed introduced into a living cell by the process of bacterial transformation. See Genetic engineering



a generic term for any intracellular hereditary factor that is not located within the chromosomes. Plasmids include genetic factors in cell organelles, for example, mitochondria and plastids, as well as genetic factors that are not found in any essential components of cells. Of the plasmids that are not associated with any permanent structure, the most extensively studied are the kappa-particle in paramecia (which produces the antibiotic paramecin), the sensitivity factor for CO2, and the agent that inhibits the occurrence of male Drosophila. In addition, several bacteria possess plasmids. Bacterial plasmids control resistance to medicines and regulate the synthesis of bacteriocin, enterotoxin, hemolysin, and certain antigens. The plasmids that are called sex factors control sexual differentiation in bacteria. It has been shown that many plasmids consist of ring molecules of double-stranded deoxyribonucleic acid with a molecular weight that ranges from 106 to 108 daltons.



An extrachromosomal genetic element found among various strains of Escherichia coli and other bacteria.
References in periodicals archive ?
To crosscheck for valid plasmid transference, several random transconjugant colonies were subcultured and confirmed for ESBL using Phenotypic Confirmatory Test.
section]) This plasmid has many missing or extra nucleotides in homopolymeric regions compared with all other related plasmids.
The researchers are using a Tet-On 3G inducible PiggyBac system that is a plasmid they named XLone to achieve insertion, activation and removal.
To characterise the plasmid complement of these bacteria in silico the plasmids were annotated to identify whether or not these plasmids encoded antibiotic resistance genes.
The first use of the pXX6 plasmid in recombinant AAV (rAAV) production was published in 1998 (J.
When resistance plasmid transfer failed in mating experiments, a transformation was used.
However Electro-transformation is considered the most reliable and efficient tool for plasmid DNA uptake.
Plasmid DNA was isolated from the multiple antibiotics resistant strains according to Birnboim and Doly (1979) and was done to separate identify and purify the plasmid DNA through agarose gel electrophoresis (Meyers et al.
By establishing this partnership, Althea will be able to leverage our plasmid manufacturing expertise to help advance this DNA vaccines program through the clinic and to the patients who need it.
lambda] ] was engineered to give high level of supercoiled (gyrA96) and more stable pDNA (recA = recombination deficient) and to avoid plasmid degradation (end Al = endonuclease I deficient) [14].