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We observed that the RHAMM immunoreactivity of uterine epithelial cells and subepithelial cells increased in proestrus compared with estrus and diestrus cycles (Table 1).
5 ppm doses increased the numbers of days the rats were in proestrus (Figure 4B), even though these treatment regimes failed to affect the number of days the rats were in estrus (Figure 4C), the number of cycles observed in the 30-day period (Figure 5), or the length of the estrous cycle (Figure 6).
This hormone is very important for the induction of the preovulatory LH surge from the pituitary during the afternoon of proestrus of each estrous cycle.
There was no effect of DPP or DBBP on serum estradiol or estrone levels measured during proestrus.
Smears were stained with Giemsa and scored for proestrus, estrus, metestrus, or diestrus according to classical vaginal smearing technique (15).
The vaginal cytology of some animals revealed intermittent extended periods of diestrus, whereas others exhibited extended periods of proestrus and/or estrus.
In a typical 4-day cycle, serum levels of estradiol, progesterone, prolactin, testosterone, and androstenedione peak during the later half of the day of proestrus (17,18).
The longer estrous cycle resulted from a longer diestrus phase, and shorter estrus and proestrus phases of the two experimental groups, as compared with the control group (p < 0.
Diestrus was identified by predominance of small cells (leukocytes) as well as larger, round, nucleated epithelial cells and cornified epithelial cells; diestrus II by leukocytes and round, nucleated epithelial cells with few cornified epithelial cells; proestrus by round, nucleated epithelial cells; and estrus by cornified epithelial cells.
For mating purposes, the phase of the estral cycle of each female rat was observed in the morning by means of a vaginal smear, classifying the phase as proestrus (12 h), estrus (14h.
Females during proestrus cycle were kept near males.
Females in proestrus stage were caged overnight with males of the same stock.
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