Pure Culture


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pure culture

[′pyu̇r ¦kəl·chər]
(microbiology)
A culture that contains cells of one kind, all progeny of a single cell.

Pure Culture

 

a culture containing cells of a single species of microorganism.

The most reliable way of isolating a pure culture is from a single cell of a population. For yeast and microscopic fungi, this is accomplished by Lindner’s drop technique. Drops from a cellular suspension of yeast or fungi spores (conidia) are transferred to a cover glass, or cover slip. The cover glass is then placed over a cavity slide. Microscopic techniques are used to find a drop containing a single cell, and the drop is then transferred, using sterile procedures, to a culture medium. Pure, single-cell bacterial cultures are isolated with the aid of a micromanipulator or thin capillaries (B. V. Perfil’ev’s microselector).

The isolation of pure cultures is necessary in the classification and study of the variability of microorganisms and in the production of vaccines. It is also used in the microbiological industry for the preparation of enzymes, antibiotics, vitamins, steroid hormones, and other preparations.

References in periodicals archive ?
In order to isolate the pure cultures of lactic acid bacteria, the plants pieces of leaves, stems, berries, and slices of vegetables were used.
fumaroxidans were higher in co-culture with methanogens than in pure culture and their transcription levels were different at different substrate, indicating that the transcription of these two formate dehydrogenase genes are dependent on growth and substrate (Worm et al.
MRSA detection in an automated setting is possible in <12 hours after pure culture with >98% sensitivity and >99% specificity.
typhimurium in pure culture is high, when in the presence of other microorganisms recovery may vary, the highest growth values were obtained in RP -this broth was productive and selective for S.
Starting with a pure culture of bacterial organisms, this novel approach relies on panbacterial real-time PCR followed by high-resolution melting analysis (14), thus offering a simple workflow and overall result turnaround times of ~1.
Founded in 2004, Pure Culture is quickly becoming an internationally recognized leader in helping a variety of businesses to build ethical, committed cultures that are aligned with a business strategy.
Subsequently, a pure culture of the epibiont was established from spatially isolated colonies on 0.
If Tanzania was anti-Bauerist in pure culture, there is no doubt that most of the countries of Africa were anti-Bauerist in their policies in greater or lesser degree.
On-farm yields of Yacine in pure culture were similar to those of Melakh (750 kg [ha.