puromycin


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puromycin

[¦pu̇r·ə¦mīs·ən]
(microbiology)
C22H29O5N7 A colorless, crystalline broad-spectrum antibiotic produced by a strain of Streptomyces.
References in periodicals archive ?
From above, cells were co-transfected with circular TALENs and linearized pBLC-TK, plated on 6-well plates and maintained at 37[degrees]C, under puromycin selection.
Keywords: MDBK-T7 cells, T7 RNA polymerase, lentivirus, stable expression, puromycin selection.
Puromycin (antibacterial/anticancer/ antitrypanosomial) is a protein synthesis inhibitor and bioisosteric analogue of aminoacyl-t-RNA.
There was no clear evidence of any differences as a result of using (a) cycloheximide vs puromycin treatment for NMD inhibition; (b) differing RNA extraction methods; (c) oligo d(T) and random hexamers vs gene specific primers; (e) various methods of DNase treatment; and (f) a particular type or brand of Taq polymerase.
Actinomycin and puromycin inhibition of cortisone-induced synthesis of hepatic glucose-6-phosphatase and fructose 1-6-diphosphatase.
Selection of infected cells was done in DMEM containing 5 [micro]g/ml puromycin for 24 hours after lipofection had been conducted for 24 hours.
Rats treated with puromycin (nephritic syndrome model) caused increased proteinuria, podocyte foot process retraction, actin cytoskeletal rearrangement, and deranged nephrin distribution.
Limitations of podocyte adaptation for glomerular injury in puromycin aminonucleoside nephrosis.
After co-transfection of the piggyBac cumate-GFP transgene vector and piggyBac transposase construct into DF1 and QM7 cells, stable cells were selected using puromycin.
To screen out stable transfected cells lines, cells were subcultured in 25 cm [sup]2 flasks with 5 [micro]g/ml puromycin (Sigma-Aldrich Co.
To induce apoptosis, BMDM were treated with cycloheximide (Sigma-Aldrich) or oxLDL (medium oxidized LDL; Kalen Biomedical; 770202) for 8 h while VSMCs were treated with puromycin (Sigma-Aldrich) or oxLDL for 16 h.