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replica plating

   Also found in: Wikipedia 0.01 sec.
replica plating [′rep·lə·kə ‚plād·iŋ]
(microbiology)
A method for the isolation of nutritional mutants in microorganisms; colonies are grown from a microorganism suspension previously exposed to a mutagenic agent, on a complete medium in a petri dish; a velour surface is used to transfer the impression of all these colonies to a petri dish containing a minimal medium; colonies that do not grow on the minimal medium are the mutants.


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When there are 500 or more beds, the workload may be great enough to also justify replica plating methods or an automated system.
The Lederbergs developed the technique of replica plating in 1952 that is still widely used in genetics laboratories.
 
 
 
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