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restriction endonuclease analysis

   Also found in: Acronyms 0.01 sec.
restriction endonuclease analysis [ri‚strik·shən ‚en·dō′nü·klē‚ās ə‚nal·ə·səs]
(cell and molecular biology)
A technique in which deoxyribonucleic acid (DNA) fragments obtained from digestion with restriction enzymes are compared to construct a restriction map showing the position of specific sites along a sequence of DNA.


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Pestiviruses isolated from pigs, cattle and sheep can be allocated into at least three genogroups using polymerase chain reaction and restriction endonuclease analysis.
However, similar patterns of genome fragments were observed among all strains following restriction endonuclease analysis using the enzyme HpaII.
difficile strain, designated NAP1 (based on its pulsed-field gel electrophoresis [PFGE] pattern), BI (by restriction endonuclease analysis [REA]), toxinotype III (by PCR characterization of the pathogenicity locus), and 027 (by PCR ribotyping) (4).
 
 
 
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