restriction fragment


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restriction fragment

[ri′strik·shən ‚frag·mənt]
(cell and molecular biology)
Any of the individual polynucleotide sequences produced by digestion of deoxyribonucleic acid with a restriction endonuclease.
References in periodicals archive ?
Resulting terminal restriction fragment (TRF) profiles were standardized for analysis using procedures similar to those described by Dunbar et al.
The restriction fragment patterns of these juveniles were identical to those of previously observed adult specimens, except for the two S.
Recent developments in national plant germplasm system; Genetic resources and utilization of the USDA Soybean Germplasm Collection; Advances in genetic alteration of soybean oil composition; Cellular and molecular biology of soybean oil storage organelles; Biochemical and physiological regulation of nitrogen metabolism in soybean; Protein synthesis and composition in high-protein soybean germplasm;' Molecular genetic control of protein composition and quality in soybean; Restriction fragment length polymorphism mapping of genes for protein and oil content in soybean; Development of high-yielding high-protein soybean germplasm; Potential commercial application of advances in soybean breeding and biotechnology; and Perspectives on the application of utility patents to plant germplasm.
Organized according to the Quality System Essentials quality template, the MT Standards addresses operational aspects such as test methods, including DNA, restriction fragment length polymorphism testing, and sequence determination or single nucleotide polymorphism (SNP) analysis.
We used variation in allozyme loci and mitochondrial DNA restriction fragment length polymorphisms to evaluate and compare the population genetic structures of bay scallops from those two regions, to propose a new interpretation for the composition of the North Carolina bay scallop population, to resolve the taxonomic quandary of Argopecten irradians taylorae, and to evaluate the apparent and potential genetic effects of the common fishery practice of hatchery-based stock enhancement on the genetic diversity and relatedness of Atlantic bay scallop populations.
The selected and interpolated traces were analyzed with custom software to identify restriction fragment maps for the sample.
Restriction fragment length polymorphism (RFLP) analysis of the PCR products of the open reading frame (ORF) 38 and ORF54 loci using PstI and BgII (5) demonstrated that the strain had a vOka-like pattern, i.
Nucleotide sequence comparison of the mycobacterial dnaJ gene and PCR restriction fragment length polymorphism analysis for identification of mycobacterial species.
Compared to currently available methods, the BeadChip Test, comprising a set of 18 genetic markers, reliably produced results equivalent to those of either serological hemagglutination or manual restriction fragment length polymorphism (RFLP) and allele-specific PCR analysis of individual markers, but in a manner substantially accelerating throughput.
6,045,994--Selective restriction fragment amplification: fingerprinting.
Chow (1994) used 13 restriction enzymes in a restriction fragment length polymorphism (RFLP) analysis of a 350-bp region of the mtDNA cytochrome b gene to discriminate among ten nominal species of billfishes; however samples sizes were small for several species and banding patterns differed by as little as 15 base pairs, making alternate patterns difficult to distinguish.

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