restriction site


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restriction site

[ri′strik·shən ‚sīt]
(cell and molecular biology)
A sequence in a deoxyribonucleic acid molecule that can be cleaved with a specific restriction endonuclease.
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Approx 30% of the genotyped animals presented additional restriction sites resulting in a new fragment.
The resultant construct, pBS-HP-D2D thus contained an introduced AatII restriction sites at the 2nd codon of HC- Pro gene as a consequence of an introduced silent mutation (Fig.
Nevertheless, the available variability of the fragment lengths is naturally very restricted due to (i) the limited distance between two restriction sites, and (ii) the usually very stringent size-selection step during library preparation.
The insertion of the KpnI restriction site allowed the circularization of the linear amplicon.
The nature of the TRFLP analysis makes definitive identification of TRFs difficult, because numerous unrelated bacteria groups may share restriction sites, and fluorochrome dye labels and purine content can influence sizing errors during analysis (Schutte et al.
The "B" allele denotes absence of the Bsm1 restriction site, while "b" allele indicates presence of the restriction site.
For [lambda] DNA sequences, we counted the number of reads mapped to each restriction site and ignored sites with restriction fragment lengths of <30 bp (because 25 bp was used for alignment).
Thus, the Oka vaccine strain was positive for the additional SmaI restriction site in ORF 62 (SmaI+), while all the circulating wild-type VZV strains in India were negative (SmaI-).
This study showed that the occurrence of a BsmI restriction site (denoted b) in the intron between exons 8 and 9 of the gene is associated with enhanced lumbar spine BMD.
This study independently applies morphological characters and a key based on mitochondrial restriction site variation to identify juvenile rockfishes collected in southern California during juvenile rockfish surveys.
The RNA transcript may be formed by linearization of the vector through cleavage at a unique restriction site in a plasmid vector and then transcribing the linear molecule.