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sequencing

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sequencing

Determining of the order of amino acids in a protein or of nucleotides in a nucleic acid or gene. The results have increased understanding of the mechanisms of life processes and have numerous applications. Whereas Frederick Sanger required 10 years to determine the structure and sequence of amino acids in insulin and about as long to determine the sequence of nucleotides in the DNA of a small virus, automated laboratory instruments and techniques can now do either task in days or hours. See also genetic code; polymerase chain reaction.


sequencing [′sē·kwəns·iŋ]
(industrial engineering)
Designating the order of performance of tasks to assure optimal utilization of available production facilities.


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Other topics include sequence data analysis, real-time DNA sequencing, a single DNA molecule barcoding method with applications in DNA mapping and molecular halpotyping, optical sequencing, the multiplex sequencing of paired end ditags for transcriptome and genome analysis, microRNA discovery and expression profiling, and ethical issues about personal genome information.
We report results of further characterization of this virus, including growth kinetics in cell culture and full-length genome sequencing and genetic characterization, which show it to be distinct from DUGV.
The most widely used technology for DNA sequencing is a capillary electrophoresis-based (CE) system employing the Sanger method.
 
 
 
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