The geographic origin of tested and EBLV-positive serotine and pond bats are depicted in Figures 4 and 5, respectively.
From 1984 to 2003, the serotine bat appeared to be the main wildlife reservoir of EBLVs in the Netherlands, as in several other European countries (7).
The difference in the overall prevalence between the serotine bat and the pond bat is difficult to explain.
The serotine bat is relatively common and can be found throughout the country.
Sequences from serotine bats with high homology for at least 2 EBLV1a lineages (clusters 2 and 4, Figure 3) originated from a defined geographic region in the Netherlands.
EBLV sequences detected in native bats in the Netherlands showed little divergence and did not indicate an emergence of new EBLV strains, but this study confirms that the serotine bat is an EBLV1 reservoir.