At the end of the learning procedure carried out on days 2, 9, 16 and 23 after the acclimatization of the rats, using the shuttle box, rats from each group were sacrificed by decapitation.
Table 1 shows the time spent by the rat in the light compartment before centering the dark compartment of the shuttle box.
There was significance in the time spent in the light compartment of the shuttle box by the albino rats fed control diet before entering the dark compartment as compared to the rats fed Zn adequate diet on days 2 and 9.
This observation suggests that the zinc adequate rat diet exhibited highest cognitive behaviour during learning and memory test using the shuttle box.
Table 1: Time spent in the light compartment before entering the dark compartment by rats fed Zn adequate diet, Zn deficient diet and control diet during training using shuttle box (1,2).
The shuttle box was divided into two halves by a stainless steel partition which had a 6- x 7-cm rounded archway cut out of it.
On the test day, there was no access to water prior to the shuttle box test.
Twenty-four hours after the training session, the animals in each group were given five unsignaled FR 1 escape trials in a shuttle box, during which crossing from one side to the other terminated shock.
Three measures were used to evaluate shuttle box escape performance: FR1 latency, FR2 latency, and number of failures.
1978), who eliminated the escape impairment by increasing aversive drive during the shuttle box escape test.