single radial immunodiffusion

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single radial immunodiffusion [¦siŋ·gəl ¦rād·ē·əl ‚im·yə·nō·də′fyü·zhən]

(immunology)

A technique for quantitating soluble proteins that involves placing the solution to be measured into a well cut into an agar or agarose gel containing antiserum specific for the protein. As the solution to be measured diffuses out of the well, it complexes with the antiserum and forms a ring, the size of which is proportional to the quantity of soluble protein in the well. Abbreviated SRID. Also known as Mancini method.

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