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The enzyme exhibited Km and Vmax values of 50 mM and 2500 umole/min respectively when sodium phytate
was used as substrate.
2% sodium phytate
and incubated at 39[degrees]C for 15 min.
to 100 lysolecithin, sclerotium gum, caprylyl glycol, chlorphenesin, pullulan, xanthan gum, sodium phytate
, silica) Phase B Syn-Star (DSM Nutritional Products/Centerchem, Inc.
Phytic acid was estimated according to Latta and Eskin  using the standard curve prepared with synthetic sodium phytate
and expressed as g/100g on dry weight basis.
One FTU is defined as the amount of enzyme that generates 1 [micro]-mole of inorganic phosphorus per min from an excess of sodium phytate
at pH 5.
1] sodium phytate
(C6H6O24P6Na12--Sigma-Aldrich) in buffered medium (0.
84 gm sodium phytate
in 90 ml acetate buffer, pH 5.
The material was incubated with (A) or without (B) 5mM of sodium phytate
, including a control without sample (C), but with sodium phytate
5; temperature 37degC; substrate: sodium phytate
in a concentration of 0.
One FTU is the amount of enzyme that liberates one micromole of inorganic phosphate per minute from a sodium phytate
substrate at pH 5.
The phytic acid content was calculated from a standard curve run using sodium phytate
(Wako Company, USA)