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instrument for measuring and comparing the intensities of common spectral lines in the spectra of two different sources of light. See photometryphotometry
, branch of physics dealing with the measurement of the intensity of a source of light, such as an electric lamp, and with the intensity of light such a source may cast on a surface area.
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; spectroscopespectroscope,
optical instrument for producing spectral lines and measuring their wavelengths and intensities, used in spectral analysis (see spectrum). When a material is heated to incandescence it emits light that is characteristic of the atomic makeup of the material.
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; spectrumspectrum,
arrangement or display of light or other form of radiation separated according to wavelength, frequency, energy, or some other property. Beams of charged particles can be separated into a spectrum according to mass in a mass spectrometer (see mass spectrograph).
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(spek-troh-foh-tom -ĕ-ter) An instrument designed to measure the intensity of a particular spectral line or a series of spectral lines in an absorption or emission spectrum.



a spectroscopic instrument by means of which photometric measurements are carried out. In spectrophotometers, the light flux being measured is compared with a standard, or reference, flux for a continuous or discrete series of radiation wavelengths. The results of the comparison may be read manually or be recorded automatically on a chart where the abscissa is the wavelength and the ordinate is the result of the photometric measurement at this wavelength.

Also classed as spectrophotometers are analytical instruments that, instead of measuring spectra, determine the concentration of elements in a sample on the basis of the absorption or emission lines of atoms in a flame (absorption or flame spectrophotometers) or determine the concentration of components in mixtures of substances on the basis of characteristic absorption bands (double-beam infrared spectrophotometers or analyzing spectrophotometers).

The principal types of spectrophotometers are described in SPECTROSCOPIC INSTRUMENTS.


An instrument that consists of a radiant-energy source, monochromator, sample holder, and detector, used for measurement of radiant flux as a function of wavelength and for measurement of absorption spectra.


An instrument for measuring the reflectance and transmittance of surfaces and media as a function of wavelength.
References in periodicals archive ?
The developed method is based on spectrophotometric detection of SOF at wavelength 262 nm using double beam spectrophotometer and quartz cells of 1 cm path length.
A sensitive, simple and selective spectrophotometric method was developed for determination of cephalexin in pure form and commercial formulation.
UV/Visible Spectrophotometric stability indicating methods for quantitative determination of cimetidine, famotidine and ranitidine HCl in prescence of their oxidative derivatives.
2] induced a significant change in chlorophyll content in mostly all cultivars determined by both extractable spectrophotometric method (SPM) and portable chlorophyll meter method (PCM) (Figure 1a-f and Fig.
The formulated problem is solved using for spectrophotometric MW catalase activity estimation the mineral water research sample volume of 5,0 ml, assigning its incubation time as 30 min; the catalase activity is calculated as a percentage.
The preparations of working standard solutions were made by appropriate dilutions from stock solution in methanol for the spectrophotometric methods and with acetonitrile-water (70: 30, v/v) for the HPLC method.
Oleay Sagirli (2000) Spectrophotometric Determination of Amlodipine Besylate and Aspartame in Tablets.
The potential application of hydrazone derivatives for the spectrophotometric determination of metal ions was reviewed by Singh [1] and others.
The antioxidant ability of plasma was estimated by the FRAP assay using the spectrophotometric method.
Reaction optimization using Fourier transform infrared spectroscopy (FTIR), mass spectroscopy (MS), nuclear magnetic resonance (NMR) methods, and spectrophotometric techniques receive much attention, within the context of building combinatorial libraries.
Drugs: Twenty tablets/capsules of NVP (Nevirex 200, Aurobindo Pharma Limited, India), 3TC (Lamivox 150, Aurobindo Pharma Limited, India), d4T (Stavir 30, Cipla, India) each were analysed for their drug content by HPLC and spectrophotometric methods.