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spectrophotometry

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spectrophotometry

Branch of spectroscopy dealing with measurement of radiant energy transmitted or reflected by a body as a function of wavelength. The measurement is usually compared to that transmitted or reflected by a system that serves as a standard. In chemistry and physics, different types of spectrophotometers cover wide ranges of the electromagnetic spectrum: ultraviolet (UV), visible light, infrared (IR), or microwave. UV spectrophotometry is particularly useful in detecting and quantifying colourless substances in solution. IR spectrophotometry is used mostly to study the molecular structures of complex organic compounds. In astronomy and astrophysics, spectrophotometric studies also include the X-ray and gamma ray ranges of the spectrum. See also colorimetry.


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RNA was then quantified by spectrophotometry, diluted serially from 100 to [10.
Method accuracy has been evaluated through comparison of XRF data from cadaver specimens with electrothermal atomic absorption spectrophotometry data (Todd et al.
A virtually instant match to any color sample or swatch would be achieved by combining color-dispensing and mixing technology with spectrophotometry, explains Chuck Morgan, product marketing manager at Conair in Pittsburgh.
 
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