spore stain

spore stain

[′spȯr ‚stān]
(microbiology)
A type of staining technique that utilizes the fact that the spore does not take up dyes readily but, once stained, it resists decolorization. The differentiating agent used may be a dilute solution of an organic acid, an acid dye, or another basic dye.
References in periodicals archive ?
Objects with possible contamination were evaluated for spores with the spore stain (Malachite green) by wet-mount-phase microscopy and were cultured for Bacillus anthracis under modified biosafety level-3 conditions (2).
A The spores of Bacillus anthracis do not fluoresce under ultraviolet light, but can be visualized by phase contrast microscopy or after staining with a spore stain.
A spore stain may be used, but often the suspected powder does not adhere to the slide.