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supernatant |
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supernatant [‚sü·pər′nāt·ənt] (biochemistry) The overlying soluble liquid fraction of a sample that remains after precipitation of the insoluble solid component by centrifugation. How to thank TFD for its existence? Tell a friend about us, add a link to this page, add the site to iGoogle, or visit webmaster's page for free fun content. |
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Although the specimen exhibited enterovirus-like cytopathic effect by inoculation into HEF and GMK cells when cell culture system for virus isolation was used (2), extracted RNA from the supernatant of the cells showed no amplification by reverse transcription--PCR (RT-PCR) when 1 of the conventional primer sets for human enteroviruses was used (3,4). The water suspension of UPs was centrifuged for 10 min at 14,000 rpm; we then collected the supernatant and centrifuged it again. N, Collman's lab showed that neurons die in response to supernatant from macrophages exposed to gp120. |
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