supernatant

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supernatant

[‚sü·pər′nāt·ənt]
(biochemistry)
The overlying soluble liquid fraction of a sample that remains after precipitation of the insoluble solid component by centrifugation.
References in periodicals archive ?
coli O157:H7 supernatant (20, 60, and then log-fold to 600 000 bacteria/mL) were injected onto anti-O157 immobilized sensors.
For calibration in plasma and cell culture supernatants, we prepared a stock solution containing 150 [micro]mol of Arg, 3 [micro]mol of ADMA, and 4 [micro]mol of SDMA in 100 mL of water.
For each reaction, we added 375 nM common primer, 25 nM of each allele-specific primer, 25 nM (TCN2 776C>G, SLC19A1 80G>A) or 50 nM (MTHFR 677C>T) of each uniprimer, and 50-100 ng of DNA or 2 [micro]L of boiled blood supernatant.
Since 5 [micro]L of 50 [micro]L RNA eluates that were derived from 560 [micro]L throat wash supernatant, was used in each reaction, the number of SARS-CoV RNA copies per reaction was divided by 56 [micro]L (560 [micro]L x 5 [micro]L/50 [micro]L) and multiplied by 1,000 to determine the RNA copies per milliliter.
Trp and Kyn concentrations were determined in the supernatants from cultures of human primary macrophages in serum-free medium with this HPLC method (Fig.
We determined the phospholipase activity of supernatants obtained from cultures of the different C.
avg] for 5 min, and supernatant was isolated as cytosolic protein fraction.
2], after which the culture supernatant was collected and stored at -70[degrees]C.
The Removal of HIV-1 from Cell Culture Supernatants, Plasma, and Blood," was published in the latest issue (Vol.
As a measure of immune function, various cytokines are currently being measured in serum/plasma samples or in tissue culture supernatants after antigen/mitogen stimulation (1-3).
WNV isolation was confirmed by immunofiuorescence using chicken anti-WNV polyclonal sera and one-step RT-PCR of infected tissue culture supernatants.