35] Dilution, filtration, and (for frozen samples) a modification of the dextran sulfate-magnesium method can each be used to eliminate triglyceride-rich particles from supernates
when using precipitation methods.
These antibodies completely neutralized the activity in the macrophage-conditioned supernate
, thus indicating that the increase in thymocyte proliferation was due to the presence of IL-1 in the macrophage-conditioned culture media.
After centrifugation, the supernate
rose into the central well, and the device could be placed directly in the sample tray of a chemistry analyzer.
The DCM uses direct dextran sulfate /magnesium precipitation followed by Abell-Kendall cholesterol analysis of the supernate
After centrifugation (10 000g for 5 min), the protein content of the supernate
(UPL stock solution, 1.
For biochemical studies, NAF samples (1-500 [micro]L) were centrifuged at 19000g for 15 min at 4[degrees]C; after removal of the top lipid layer, the clear supernate
was analyzed for free and total PSA content (expressed as [micro]g/L) by a commercially available kit based on a microparticle enzyme immunoassay with a mouse anti-human monoclonal antibody (AxSYM[R]; Abbott Laboratories).
That is, HDL-C generally is quantified as the cholesterol remaining in the supernate
after chemical precipitation and sedimentation of other lipoproteins.
2], followed by quantification of the cholesterol content in the HDL-containing supernate
After centrifugation (1500g for 15 min), the supernate
was divided into aliquots of 2 mL in polystyrene tubes.
The cholesterol content of the HDL particles that remain in the supernate
can easily be measured by the CHOD-PAP method.
The first extraction was carried out for 24h and the homogenized was filtered and the supernates
stored in the refrigerator at 4[degrees]C.
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