supernatant

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supernatant

[‚sü·pər′nāt·ənt]
(biochemistry)
The overlying soluble liquid fraction of a sample that remains after precipitation of the insoluble solid component by centrifugation.
References in periodicals archive ?
35] Dilution, filtration, and (for frozen samples) a modification of the dextran sulfate-magnesium method[36] can each be used to eliminate triglyceride-rich particles from supernates when using precipitation methods.
These antibodies completely neutralized the activity in the macrophage-conditioned supernate, thus indicating that the increase in thymocyte proliferation was due to the presence of IL-1 in the macrophage-conditioned culture media.
After centrifugation, the supernate rose into the central well, and the device could be placed directly in the sample tray of a chemistry analyzer.
The DCM uses direct dextran sulfate /magnesium precipitation followed by Abell-Kendall cholesterol analysis of the supernate.
After centrifugation (10 000g for 5 min), the protein content of the supernate (UPL stock solution, 1.
For biochemical studies, NAF samples (1-500 [micro]L) were centrifuged at 19000g for 15 min at 4[degrees]C; after removal of the top lipid layer, the clear supernate was analyzed for free and total PSA content (expressed as [micro]g/L) by a commercially available kit based on a microparticle enzyme immunoassay with a mouse anti-human monoclonal antibody (AxSYM[R]; Abbott Laboratories).
That is, HDL-C generally is quantified as the cholesterol remaining in the supernate after chemical precipitation and sedimentation of other lipoproteins.
After centrifugation (1500g for 15 min), the supernate was divided into aliquots of 2 mL in polystyrene tubes.
The cholesterol content of the HDL particles that remain in the supernate can easily be measured by the CHOD-PAP method.
The first extraction was carried out for 24h and the homogenized was filtered and the supernates stored in the refrigerator at 4[degrees]C.
a leading provider of immunoassay kits, antibodies, and reagents to the life science and translational research markets, has introduced a new line of MultiBead multiplex immunoassays to simultaneously quantify the levels of up to 22 analytes in a single sample and in various matrices including serum, plasma, urine, culture supernates, and cell lysates.