In this study, deoiled rice bran, a locally available industrial waste, was used as a substrate for the production Tannase and analysis the phenolic compounds in fermented substrate by using HPLC
The supernatant was collected and estimate the Tannase activity.
The crude tannase was precipitated by adding Solid Ammonium sulphate (60-80%) and kept for overnight at 4[degrees]C.
Production of Tannase from Rice bran fermented with Aspergillus niger was shown in Fig-1: The maximum production of was obtained at 96 h of incubation at 30[degrees]C in purified form.
Jackson and Lee believe their findings suggest the prospect of reducing some teas' detrimental effects on mineral bioavailability, perhaps with something like a tannase
Most of the reports are pertaining to extracellular tannase (ECT) production from bacteria and fungi .
Tannase activity was estimated by reaction with tannic acid as substrate followed by the reaction with methanolic rhodanine as described elsewhere .
The supernatant was taken to find soluble intracellular tannase activity.
CAT showed lesser stability at 50[degrees]C and above, which is in agreement with the previous findings in case of mycelium bound tannase of Aspergillus niger .
This study was taken up with the objective to produce increased quantities of tannase through bioconversion of groundnut shell as a cheaper raw material, using fungal organisms.
The supernatant (mycelia extract) was used for tannase assay.
A volume each of 100 ml of crude tannase was taken, added slowly with the various concentration levels (0-40, 40-60 and 60-80 %) of ammonium sulphate.