Current bench-based, fluorometric enzyme assay protocols require 2 separate punches for measuring the activity of GAA and GLA, because the punch for Pompe assay is extracted in deionized water, whereas the punch for Fabry assay requires 1% sodium taurocholate
On enrollment, fresh stool sample was taken from each patient for culture (inoculated into tellurite taurocholate
gelatin agar, Mackonkey and Shigella-Salmonella agar) and sensitivity test.
reduced the activity of all 3 enzymes at all concentrations tested (see Table 3 in the online Data Supplement).
OATP-B, OATP-D, OATP-E) tended to have substrate affinity toward steroid sulfate, although OATP-E (an Oatp4a1 homolog) also had affinity toward taurocholate
Briefly, 5 g of each sample was cultured in 100 mL of prereduced brain-heart infusion (BHI) broth supplemented with cefoxitin (10 [micro]g/mL), cycloserine (250 [micro]g/mL), and taurocholate
5 [micro]g/min for 60 min in 140 mM NaCl containing 10mM sodium taurocholate
using a Harvard syringe pump.
In the early period after hatching, the bird entero-hepatic circulation is still immature, and this causes the accumulation of taurocholate
in ileum (JEANSON & KELLOGG, 1992).
0 gm % sodium taurocholate
to the standard (basal) commercial diet and daily feed consumption was recorded.
Merck, Darmstadt, Germany (all substances not specifically attributed to other source), Novartis, Grenzach-Wyhlen, Germany (Liquemin[R]), Sigma, Munchen, Germany (BSA, sodium taurocholate
and thiobarbituric acid) and Synopharma, Hamburg, Germany (butylhydroxytoluol).
To the residue we added 15 [micro]L of 120 g/L sodium taurocholate
Cyp7b1 is the hydroxylase involved in taurocholate
bile acid synthesis in mice (Russell 1999).
Cyclooxygenase and lipoxygenase metabolism in sodium taurocholate
induced acute hemorrhagic pancreatitis in rats.