They told us what they'd' like to see in a transilluminator
Viewed the gels on UV transilluminator
and photograph of the gel was taken (Sudip Kumar Das et al.
The PVDF membrane was viewed and photographed under a UV light transilluminator
The RT-PCR product, which was stained with GelRed[TM] (Biotium), was visualised in a 1% agarose gel using a UV transilluminator
8% agarose gel in TAE buffer (Tris-Acetate-EDTA), with an electrophoretic run carried out at a constant voltage of 100V/75, stained with ethidium bromide for 20 minutes, visualized on a transilluminator
Several lines of evidence suggest, however, that the retention of the tourniquet for more than one to two minutes is a significant cause of haemoconcentration, which may also spuriously increase the concentration of several analytes (36,37), but which can be avoided by using transilluminator
After development, the gels were evaluated using a transilluminator
, and the presence or absence of bands and the relative intensity of the different isoforms for each sample was determined.
This, combined with an integral slide-out UV transilluminator
, and optional blue epi-illumination module and white light table, make the system suitable for documentation and analysis of almost all fluorescent and colorimetric gels.
Table 1 Electronic recyclers commonly process these types of medical and lab equipment: Anesthesia Unit Electrocardiograph Autoclave Endoscope Blood Analyzer Freeze Dryer Cast System Gas Chromatograph Centrifuge Goniometer Concentrator Glucose Meter Defibrillator Incubator Dialysis Unit IV Pump Drying Oven Microcontroller Microscope Spectrometer Na+/K+Analyzer Ultrasonic Cleaner Ophthalmoscope Ultrasound Equipment Otoscope UV Transilluminator
Oxygen Monitor UV Disinfection System PH Meter UV Meter Pulse Oximeter UV Spectrophotometer Sample Oxidizer Water Test Kit Shaker Vitals Monitor
The amplification products were analysed on 2% agarose gels (Fisher Scientific, India] using a 100 bp DNA ladder (Promega Co, USA], visualized on the UV- light transilluminator
Firereader (UVItec, UK] and then photographed.
Step 4: After completion of electrophoresis, the molecules in the gel were stained with Ethidium bromide and the gel was placed on a UV transilluminator
for nucleic acid visualization and analysis.
The PCR product was visualized under an ultraviolet transilluminator
(TransilluminatorFBTI 88; FisherBiotech[R]).