Anaesthetic efcacy of clove oil, benzocaine, 2-phenoxyethanol and tricaine
methanesulfonatein juvenile marbled spinefoot (Siganus rivulatus).
The most commonly used anesthetics for fish are tricaine
methanesulfonate (MS-222), benzocaine, metomidate, 2-phenoxyethanol and quinaldine.
Until recently, synthetic products such as tricaine
methanesulfonate (MS 222), a mixture of ketamine/diazepam (HERNANDEZ et al.
Individual fish were removed, euthanized by exposure to neutral buffered tricaine
methane sulfonate (MS-222) followed by pithing, and dissected.
After the period of experimentation the fishes-were sacrificed with an overdose of the anesthetic drug Tricaine
Methanesulfonato MS-222 (Finquel[R]) dissolved in water.
At the end of the experiment, after fish were starved for 12 hours, randomized two fish per tank that were anesthetized with tricaine
methane sulfonate solution (MS222; Torreilles and others 2009).
By prolonged immersion in tricaine
methane sulfonate (MS222) the specimens were killed in accordance with the Manual Handling of Animals for Experiments and Education of Falconi et al.
As a positive control, from stock solution of tricaine
methanesulfonate (1mg/ml) serial dilutions were made in the range of 0.
Larvae that were collected for laboratory analysis were euthanized with tricaine
methanesulfonate (MS-222) mixed in seawater (90 g/mL) and preserved in either 95% ethanol (for otolith analysis) or 10% formalin (for gut content analysis).
Evaluation of Different Tricaine
(MS-222) Concentrations on the Transport of Juvenile Peruvian Grunt (Anisotremus scapularis)
As regards anesthesia, the dilution of buffered anesthetic agents like tricaine
ethanesulfonate or MS222 and benzocaine hydrochloride directly into the water is generally recommended (CONCEA, 2013a).