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tris buffer |
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The sections were incubated in a substrate solution containing the mixture of L-leucyl-bnaphthylamide, distilled water, tris buffer and garnet GBC for 1h at 37[degrees]C, then in tap water for 5 min and counterstained by Hematoxylin. The ammonium carbonate buffer system demonstrated a significant improvement over the Tris buffer system and was selected for use in the speciation of six arsenic species in urine. One-gram portions of the icing from the top of the wedding cake, the raspberry filling, and the cake itself were processed for molecular analyses by using 20 mL of Tris buffer (containing 100 mM Tris-HCL, pH 8. |
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